Using Ion Exchange Chromatography to Separate Proteins

Ellyn A. Daugherty, Access Excellence at the National Health Museum Activities Exchange

When last checked this resource was offline Our automated link checker has alerted the folks responsible for the part of our site where this problematic link is referenced. If you have further information about the link (e.g. a new location where the information can be found) please let us know.

You may be able to find previous versions at the Internet Archive.

This activity website, hosted by the National Health Museum Activities Exchange, explores protein purification using ion chromatography. Students will separate a positively charged lysozyme from a negatively charged albumin at a neutral pH using ion exchange chromatography. The site includes information for the teacher, instructions and follow-up questions for students, and a list of supplies.

This resource is referenced here:
Subject: Biology, Biogeochemistry, Microbiology, Geoscience:Geology:Geochemistry:Organic and Biochemistry
Resource Type: Activities:Lab Activity
Grade Level: College Upper (15-16), High School (9-12), College Lower (13-14)
Topics: Biosphere:Biogeochemistry, Microbiology, Solid Earth:Geochemistry:Organic and Biochemistry, BiosphereKeywords: anion, cation, column chromatography